Muscle blood flow was assessed by contrast enhanced ultrasound (Sonovue). Bolus achieved rapid insulinemia (12.7 iU/ml 25 min postfeed), essential aminoacidemia (3,000 M, 45 “65 min postfeed), and mTORC1 activity; pulse achieved attenuated insulin responses, gradual low amplitude aminoacidemia (1,800 M 80 “195 min after feeding), and undetectable mTORC1 signaling. Despite this, equivalent anabolic responses were observed: fasting FSRs of 0.051 and 0.047%/h (bolus and pulse, respectively) increased to 0.084 and 0.073%/h steroids, respectively.
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